Journal: Pharmaceutics

Article Title: Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin

doi: 10.3390/pharmaceutics15041049

Figure Lengend Snippet: Production of angiogenic cytokines in mMSCs. ( a ) Representative microscopic images of the mMSCs. Left phase contrast image. Original magnification ×40. Right, red fluorescence indicates PDGFR-α. PDGFR-α was ubiquitously expressed in the mMSCs. Nuclei were stained with DAPI (blue). Bar = 50 µm. ( b ) Concentration levels of VEGF and HGF in the conditioned medium. N = 6. * p < 0.05 vs. BMMSCs. # p < 0.01 vs. skMCs. mMSCs, mesenchymal stem/stromal cells derived from skeletal muscle; PDGFR-α, platelet-derived growth factor receptor-α; DAPI, 4’,6-Diamidino-2-phenylindole; VEGF, vascular endothelial growth factor; HGF, hepatocyte growth factor; BMMSCs, bone marrow mesenchymal stem/stromal cells; skMCs, skeletal muscle cells.

Article Snippet: Rat skeletal muscle cells (skMCs) and human umbilical cord vein endothelial cells (HUVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA).

Techniques: Fluorescence, Staining, Concentration Assay, Derivative Assay

Journal: Pharmaceutics

Article Title: Mesenchymal Stem/Stromal Cells in Skeletal Muscle Are Pro-Angiogenic, and the Effect Is Potentiated by Erythropoietin

doi: 10.3390/pharmaceutics15041049

Figure Lengend Snippet: Proliferation of cultured mMSCs by Erythropoietin (Epo). ( a ) Left, Epo-R (green) expression in mMSCs. Nuclei were stained with DAPI (blue). NC, negative control. Bar = 50 µm. Right, western blot analysis. Epo stimulated the phosphorylation of Akt and STAT3 in the mMSCs. Epo-R, erythropoietin receptor. ( b ) Efficacy of Epo stimulation on the propagation of HUVECs, skMCs, and mMSCs. N = 3 to 6. # p < 0.01. HUVECs, human umbilical cord vein endothelial cells.

Article Snippet: Rat skeletal muscle cells (skMCs) and human umbilical cord vein endothelial cells (HUVECs) were purchased from Cell Applications Inc. (San Diego, CA, USA).

Techniques: Cell Culture, Expressing, Staining, Negative Control, Western Blot, Phospho-proteomics

Journal: Scientific Reports

Article Title: Novel 1, 4-dihydropyridines for L-type calcium channel as antagonists for cadmium toxicity

doi: 10.1038/srep45211

Figure Lengend Snippet: Calcium overload-preventing activity (IC 50 ) of novel synthesized compounds in A7r5 and SH-SY5Y cells.

Article Snippet: The A7r5 (rat aorta smooth muscle cells) and SH-SY5Y (human neuroblastoma cell line) were obtained from the National Centre for Cell Science, Pune, India.

Techniques: Activity Assay, Synthesized

Journal: Scientific Reports

Article Title: Novel 1, 4-dihydropyridines for L-type calcium channel as antagonists for cadmium toxicity

doi: 10.1038/srep45211

Figure Lengend Snippet: The effect of a 1 hr Amlodipine (AD) pre-treatment on CdCl 2 treated A7r5 cells represented curve with purple color. The effect of a 1 h 5a compound pre-treatment on CdCl 2 treated A7r5 cells represented curve with green color. The effect of a 1 hr 5e compound pre-treatment onCdCl 2 treated A7r5 cells represented curve with yellow color.

Article Snippet: The A7r5 (rat aorta smooth muscle cells) and SH-SY5Y (human neuroblastoma cell line) were obtained from the National Centre for Cell Science, Pune, India.

Techniques: